How to Test THC Content? - Leaf Nation (2024)

As the cannabis industry expands, more and more growers are cultivating the cannabis flower. However, to get from seedling to the finished cannabis product that we can buy at the local dispensary, the product needs to pass several mandatory tests and regulation procedures. Under the regulations of the FDA, producers need to list the cannabinoid profile on the label of their product (for example, the total THC composition), although sometimes the number on the label doesn’t match the actual THC potency of the cannabis product.

But is it possible to determine how much THC is present in your cannabis strain from the comfort of your own home? While there is no official industry standard for testing THC in cannabis products, the most accurate method to determine THC % in the products is using THC test kits. Below, we’ll go over all the information you need on cannabis testing kits and how you can test your cannabis samples at home.

Cannabis and Its Main Cannabinoids (THC and CBD) and Terpenes

Before we go over how you can test your cannabis samples, we need to give you the essential information on cannabis, its cannabinoids, and terpenes.

The cannabis plant is made from a variety of chemicals like hydrocarbons, amino acids, terpenes, sugars, and cannabinoids. Terpenes like myrcene, limonene, caryophyllene, humulene, and others create the scent that’s associated with distinctive weed strains, while the cannabinoids like THV, CBC, CBG, CBD, and CBN, interact with the body’s endocannabinoid system.

Even though they’re all essential for the plant, scientists are mostly interested in the main cannabinoids found in weed – tetrahydrocannabinol (THC) and cannabidiol (CBD). The former has psychoactive effects on users, while the latter provides therapeutic, anti-inflammatory, and pain-relieving benefits which is why it’s mostly used by medical marijuana patients. Through the process of decarboxylation, the tetrahydrocannabinolic acid (THCA) and the cannabidiolic acid (CBDA) turn into THC and CBD which, once consumed, give users their desired effects.

The Importance of Testing THC Levels in Cannabis

Testing for the cannabinoid profile of a specific sample or strain is essential for determining the quality of the marijuana product, especially for medical marijuana patients who need exact doses. Because there’s a huge variety of cannabis strains on the market today, more focus should be put on testing samples to determine the exact percentage of cannabinoids that the strain contains.

Since the intensity of the strain depends on the most influencing cannabinoids, THC and CBD, their ratio contributes to the potency of the sample. If the THC content is too high, users may experience side effects like euphoria, minor hallucinations, panic attack, or other effects, so it’s essential that the dosing recommendations are precise. Doctors especially recommend testing the medical cannabis samples for medical marijuana patients before they start using them.

Laboratory Methods for Testing THC

Different accredited cannabis testing labs may provide different testing options, and the most common ones include:

  • High Performance Liquid Chromatography (HPLC) – HPLC quantifies the THC and CBD content as well as other cannabinoid concentrations based on separation, identification, and analysis of the components in the mixture.
  • Gas Chromatography (GC) and Liquid Chromatography (LC) – Chromatography allows for the separation of small molecules, proteins, or nucleic acids by boiling the molecules.
  • Thin Layer Chromatography (TLC) – TLC is used to test flowers, hash, and edibles, however, it won’t work with products that contain glycerin.

Apart from testing for the cannabinoid content, laboratories may also test whether the cannabis is free from pests, pesticides, mold, mildew, and chemicals which is essential for people with compromised immune systems or severe illness.

How Can You Test THC Levels in Cannabis at Home?

THC potency testing can even be done in the comfort of your own home by using a home THC test kit. To do the test, you can follow the steps we’ve provided for you below.

Purchase a Home Test Kit

You can find a huge variety of testing kits either in your local dispensary, or you can order one online. Choose a kit that looks for THC in particular, since kits that test for both THC and CBD might be more difficult to interpret. The price will vary between $20-$100 which will determine the level of precision.

Choose Your Marijuana Strain

While a lot of marijuana strains will have the cannabinoid content provided by the vendors, home testing is always a good idea to check whether the label matches the actual THC content in the strain. When testing, make sure the strain that you’re planning to test doesn’t get in contact with other strains, as that could give you a different reading.

Read the Instructions

Although home test kits are fairly simple to use, you can find small differences between them, so it’s always a good idea to carefully read the instruction booklet. That will ensure you know exactly how to conduct the test and will decrease the chance of inaccurate results. One kit can contain several tests, so if you’re unsure whether or not you’ve gotten the results right, you could retake the test again.

Place the Marijuana in the Test Vial

Break up large clumps of weed into smaller pieces and place about one-fifth of a gram of weed in the bottom of the vial. Make sure you look at the specifications of the kit so you know exactly how much you should put in the vial.

Add the Testing Solution

By acting as a solvent, the testing solution will draw out the components in the cannabis so they’ll be easier to identify. Add a few drops of the solution to the vial (use as much as the instructions specify) on top of the weed.

Close and Shake the Vial

After placing both the weed and the solution, close the vial and check that it’s properly sealed. Shake it for around 5-10 seconds and prop the vial so it stays vertically and the sample stays at the bottom.

Wait for the Sample to React

The instructions will provide the time needed to get the results from the testing (they should be around 10 minutes). After a few minutes, the solution will slowly start to change colors. A more intense color will indicate a higher level of a certain cannabinoid.

Check the Sample Against the Color Chart

Each test comes with an included color chart which is somewhere between the instructions. By comparing the color of the vial and the chart, you’ll be able to tell the approximate concentration of THC of the weed strain you’re testing. Color charts indicate a range of potency and not the exact percentage, so if you’re looking at the exact cannabinoid profile, you should test the sample at a lab.

Conclusion

While a home testing kit is an economical option, keep in mind that it may not be 100% accurate since it only gives a rough idea of the cannabinoid profile of your sample. If you decide to test your weed at home, make sure you follow the instructions provided on the package of the kit.

However, if you’re planning to use cannabis for medical purposes, it would be best to do the testing at an accredited lab. They will also check the sample for mods, bacteria, pesticides, and other types of contaminants that can be a serious health concern for patients with compromised immune systems and sensitivities.

How to Test THC Content? - Leaf Nation (2024)

FAQs

Is there a way to test THC percentage? ›

Two NIJ-supported labs addressed this problem using different types of mass spectrometry that can measure the exact amount of THC present in a sample: gas chromatography-mass spectrometry (GC-MS) and direct analysis in real time-high-resolution mass spectrometry (DART-HRMS).

What percentage of THC is in leaves? ›

The major psychoactive content expressed as total THC decreases in the order of inflorescences (10–12%), leaves (1–2%), stems (0.1–0.3%), roots (<0.03%), and seeds (generally absent)29. As such, female flower tops are harvested while other parts are often discarded by growers29.

How do you read a THC content label? ›

Really only focus on THC, CBD, and THCA.

(For example, a product label may read “. 09% THC, 20% THCA.” Essentially, this means it contains 20% THC.) Remember: High-THC and THCA products (15% THC or higher) will have powerful mind-altering affects and will get a consumer higher.

What strain has the highest percentage of THC? ›

If you want the highest of them all, then it's even easier; the marijuana strain you need to try is Godfather OG[1], with its 34.04% THC percentage.

How to measure potency of a drug? ›

Potency testing involves comparison of a product's biological activity to that of a reference preparation and cell-based potency assays are central tools used to measure drug efficacy during potency testing. They allow scientists to see how a particular dose of a drug will react in a given biological system.

Is the tCheck accurate? ›

Seems accurate for dry herb and extracts. Not so much for infusions. I bought this from the company directly last year. The percentages for dry herb and concentrates seems to be fairly accurate when compared to results from the thin-layer chromatography test kit I have.

What do big fan leaves mean? ›

Are big fan leaves a good sign? The health of your entire hemp plant is reflected in the condition of your fan leaves. Big, green, healthy fan leaves are a sign of a robust, healthy plant.

Do pistils get you high? ›

However, pistils do not contain THC. These parts of the plant are simply there to help the plant reproduce and grow. They don't have any psychoactive properties, and they don't get you high. What does make your bud more potent, though, is a higher amount of trichomes.

What is Cinderella 99? ›

Cinderella 99 is a sativa-dominant hybrid strain that is known for her potent euphoric cerebral effects and sweet fruity flavors. Patients often report feeling dreamy and uplifted after use. This strain averages 18-22% THC, but the THC only tells a small portion of this strain's story.

What strain gets you high the longest? ›

Our Top Picks: The Best Cannabis Strains for a Long-Lasting High
  • Blissful Wizard – Top Energizing Hybrid for long-lasting high.
  • Durban Poison – People's Choice for Longer Effects.
  • Pink Gorilla – Delicious Indica Quality.
  • GMO Cookies – Powerful Pain & Stress Relief.
  • Kansas City Kush – Top Shelf Indica for Powerful Effects.

How early can I sample buds? ›

Using our listed flowering time as a guide, begin taking small samples from your topmost buds one to two weeks prior to this. Examine the bud under 100x magnification to examine the colour of the mushroom shaped trichomes. Translucent glands are immature and will not have fully developed their cannabinoid co*cktail.

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